Coding

Part:BBa_K5261015:Design

Designed by: Yutong Gao   Group: iGEM24_HiZJU-China   (2024-09-29)


FLO11, an anchoring protein for cell adhesion


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 75
    Illegal SpeI site found at 3142
    Illegal PstI site found at 3989
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 75
    Illegal SpeI site found at 3142
    Illegal PstI site found at 3989
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 88
    Illegal XhoI site found at 4020
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 75
    Illegal SpeI site found at 3142
    Illegal PstI site found at 3989
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 75
    Illegal SpeI site found at 3142
    Illegal PstI site found at 3989
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1016
    Illegal SapI site found at 1052
    Illegal SapI site found at 1097
    Illegal SapI site found at 1133
    Illegal SapI site found at 1169
    Illegal SapI site found at 1214
    Illegal SapI site found at 1250


Design Notes

Codon optimisation



Source

The FLO11 gene comes from yeast.

References